Rabbit Anti- Human 2C6 scFv Monoclonal Antibody (Clone 1F5), PE (100 tests)

Description

The rabbit monoclonal antibody 1F5 specifically binds to the scFv region of a CD20-specific human monoclonal antibody (mAb, clone 2C6). CD20 (cluster of differentiate 20) is a protein that is expressed on the surface of B cells, starting at the pre-B cell stage and also on mature B cells in the bone marrow and in the periphery. CD20 is not expressed on hematopoietic stem cells, pro-B cells, or normal plasma cells. The scFv region of 2C6 has been used to develop CD20-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-CD20 CAR expression on human cell line Jurkat cells. Jurkat cells were transduced with lentivirus encoding anti-CD20 CAR and cultured. 2×10⁵ cells were stained for the expression of anti-CD20 CAR with Rabbit Anti-Human 2C6 scFv Monoclonal Antibody, PE (Product No. 202406, right panel). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).

Product Infomation

Preparation & Storage

• Store undiluted at 2-8°C.
• Avoid prolonged exposure to light.
• The monoclonal antibody was purifie d by Protein A.
• The antibody was conjugated with PE under optimum conditions, and unincorporated dye was removed.