Rabbit Anti- Human 2C6 scFv Monoclonal Antibody (Clone 1F5) (100 μg (2mg/mL))

Description

The rabbit monoclonal antibody 1F5 specifically binds to the scFv region of a CD20-specific human monoclonal antibody (mAb, clone 2C6). CD20 (cluster of differentiate 20) is a protein that is expressed on the surface of B cells, starting at the pre-B cell stage and also on mature B cells in the bone marrow and in the periphery. CD20 is not expressed on hematopoietic stem cells, pro-B cells, or normal plasma cells. The scFv region of 2C6 has been used to develop CD20-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-CD20 CAR expression on human cell line Jurkat cells.Jurkat cells were transduced with lentivirus encoding anti-CD20 CAR and cultured. 2×10⁵ cells were stained for the expression of anti-CD20 CAR with Rabbit Anti-Human 2C6 scFv Monoclonal Antibody (Product No. 202422, right panel). Secondary staining was carried out with AffiniPure F(ab’)2 Fragment Goat anti-Rabbit IgG(H+L), Alexa Fluor 647 (Product No. 700002). Nontransduced Jurkat cells were used as a control for gating of CAR expression (left panel).

Product Infomation

Preparation & Storage

• Store at -20°C in small aliquot for long term storage. Avoid freeze/thaw cycle of the reagent
• Shipped at 2-8°C. Store at 2-8°C for short term (1 month).
• The monoclonal antibody was purified by Protein A.