Anti-CD20 CAR (2C6) Idiotype Monoclonal Antibody (Clone 1F5-RHC), hIgGC (100 μg)

Description

1F5-RHC contains a specifically mutated human IgG1 Fc region which results in a reduction in binding to Fc receptors. 1F5-RHC specifically binds to the scFv region of a CD20-specific human monoclonal antibody (mAb, clone 2C6). CD20 (cluster of differentiate 20) is a protein that is expressed on the surface of B cells, starting at the pre-B cell stage and also on mature B cells in the bone marrow and in the periphery. CD20 is not expressed on hematopoietic stem cells, pro-B cells, or normal plasma cells. The scFv region of 2C6 has been used to develop CD20-specific chimeric antigen receptor(CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-CD20 CAR expression on human cell line Jurkat cells.Jurkat cells were transduced with lentivirus encoding anti-CD20 CAR and cultured. 2×10⁵ cells were stained for the expression of anti-CD20 CAR with Anti-Human 2C6 scFv Recombinant Antibody, hIgGC (Product No. 202719, right panel). Secondary staining was carried out with Goat anti-Human IgG+IgM (H+L),Alexa Fluor 647(Product No. 800002). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).

Product Infomation

Preparation & Storage

• Store at -20°C in small aliquot for long term storage. Avoid freeze/thaw cycle of the reagent
• Shipped at 2-8°C. Store at 2-8°C for short term (1 month).
• The monoclonal antibody was purified by Protein A.