Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (Clone R2L), Alexa Fluor 647 (100 tests)

Description

The rabbit monoclonal antibody R2L specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-CD19 CAR expression on human T cells. Human T cells were transduced with lentivirus encoding anti-CD19 CAR and cultured for 7 days. 2×10⁵ cells were stained for the expression of anti-CD19 CAR with Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R2L), Alexa Fluor 647 (Product No. 203402, right panel). Non-transduced T cells were used as a control for gating of CAR expression (left panel).

Preparation & Storage

• Store undiluted at 2-8°C.
• Avoid prolonged exposure to light.
• Avoid freeze/thaw cycle of the reagent.
• The monoclonal antibody was purified by Protein A.
• The antibody was conjugated with Alexa Fluor 647 under optimum conditions, and unincorporated dye was removed.