Anti-Mouse FMC63 scFv Monoclonal Antibody (Clone R2L-RHC), hIgGC (100 μg)

Description

R2L-RHC specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-CD19 CAR expression on human cell line Jurkat cells. Jurkat cells were transduced with lentivirus encoding anti-CD19 CAR and cultured. 2×10⁵ cells were stained for the expression of anti-CD19 CAR with Anti-Mouse FMC63 scFv Monoclonal Antibody (R2L-RHC), hIgGC (Product No. 211919, right panel). Secondary staining was carried out with Goat anti-Human IgG+IgM (H+L), Alexa Fluor 647(Product No. 800002). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).

Product Infomation

Preparation & Storage

• Store at -20°C in small aliquot for long term storage. Avoid freeze/thaw cycle of the reagent
• Shipped at 2-8°C. Store at 2-8°C for short term (1 month).
• The monoclonal antibody was purified by Protein A.