Rabbit Anti-G4S Linker Monoclonal Antibody (Clone S13), Alexa Fluor 647 (100 tests)

Description

This antibody reacts with cell membrane-expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain. The poly-Glycine-Serine (G4S) linker is a kind of synthetic peptide linker sequence that is flexible and unstructured. It is frequently used to link the variable heavy (VH) domain and the variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that have an extracellular domain scFv for recognizing target antigens. The linker is made up of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and usually seen as either a 15-mer (G4S) or 20-mer (G4S) in scFv-based CARs and scFv fragments.

Flow cytometric analysis of a mixed population containing live wild-type Jurkat cells and Jurkat cells engineered to express an scFv-based Anti-CD19 CAR containing a G4S linker. Jurkat cells were transduced with lentivirus encoding anti-CD19 CAR containing a G4S linker and cultured for 7 days. 2×10⁵ cells were stained for the expression of Rabbit Anti-G4S Linker Monoclonal Antibody, Alexa Fluor 647 (Product No. 212302, right panel). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).

Product Information

Preparation & Storage

• Store undiluted at 2-8°C.
• Avoid prolonged exposure to light.
• Avoid freeze/thaw cycle of the reagent.
• The monoclonal antibody was purified by Protein A.
• The antibody was conjugated with Alexa Fluor 647 under optimum conditions, and unincorporated dye was removed.