Rabbit Anti-Leu16 Monoclonal Antibody (Clone L16-1G12), Alexa Fluor 647 (25 tests)

Description

The rabbit monoclonal antibody L16-1G12 specifically binds to the scFv region of a CD20-specific mouse monoclonal antibody (mAb, clone Leu16). CD20(cluster of differentiate 20) is a protein that is expressed on the surface of B cells, starting at the pre-B cell stage and also on mature B cells in the bone marrow and in the periphery. CD20 is not expressed on hematopoietic stem cells, pro-B cells, or normal plasma cells. The scFv region of Leu16 has been used to develop CD20-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-CD20 CAR expression on human cell line Jurkat cells. Jurkat cells were transduced with lentivirus encoding anti-CD20 CAR and cultured. 2×10⁵ cells were stained for the expression of anti-CD20 CAR with Rabbit Anti-Leu16 Monoclonal Antibody, Alexa Fluor 647 (Product No. 219201, right panel). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).

Product Infomation

Preparation & Storage

• Store undiluted at 2-8°C.
• Avoid prolonged exposure to light.
• Avoid freeze/thaw cycle of the reagent.
• The monoclonal antibody was purified by Protein A.
• The antibody was conjugated with Alexa Fluor 647 under optimum conditions, and unincorporated dye was removed.