Mouse Anti-Mouse FMC63 scFv Monoclonal Antibody (Clone M19H) , Alexa Fluor 647 (100 tests)

Description

The mouse monoclonal antibody M19H specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-CD19 CAR expression on human cell line Jurkat cells. Jurkat cells were transduced with lentivirus encoding anti-CD19 CAR and cultured. 2×10⁵ cells were stained for the expression of anti-CD19 CAR with Mouse Anti-Mouse FMC63 scFv Monoclonal Antibody, Alexa Fluor 647 (Product No. 300402, right panel). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).

Product Information

Preparation & Storage

• Store undiluted at 2-8°C.
• Avoid prolonged exposure to light.
• Avoid freeze/thaw cycle of the reagent.
• The monoclonal antibody was purified by Protein A.
• The antibody was conjugated with Alexa Fluor 647 under optimum conditions, and unincorporated dye was removed.