Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (1 mg)

Description

The rabbit polyclonal antibody R19P specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-CD19 CAR expression on human cell line Jurkat cells. Jurkat cells were transduced with lentivirus encoding anti-CD19 CAR and cultured. 2×10⁵ cells were stained for the expression of anti-CD19 CAR with Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Product No. 500028, right panel). Secondary staining was carried out with AffiniPure F(ab’)2 Fragment Goat anti-Rabbit IgG(H+L), Alexa Fluor 647 (Product No. 700002). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel). Acquisition of >10, 000 events was performed.

Product Information

Preparation & Storage

• Store at -20°C in small aliquot for long term storage. Avoid freeze/thaw cycle of the reagent.
• Shipped at 2-8°C. Store at 2-8°C for short term (1 month).
• The polyclonal antibody was purified by Protein A.