Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin (100 tests)

Description

The rabbit polyclonal antibody specifically binds to the scFv region of a B-cell maturation antigen (BCMA) specific mouse monoclonal antibody (mAb, clone C11D5.3). BCMA is a protein that has been reported to be selectively expressed by B-lineage cells including multiple myeloma cells and restrictively expressed in both normal and malignant plasma cells at high levels. The scFv region of C11D5.3 has been used to develop BCMA-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-BCMA CAR expression on Jurkat cells. Jurkat cells were transduced with lentivirus encoding anti-BCMA CAR and cultured. 2×10⁵ cells were stained for the expression of anti-BCMA CAR with Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin (Product No. 500514, right panel). Secondary staining was carried out with Streptavidin PE (Product No.700032). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel). Acquisition of >10, 000 events was performed. 

Product Infomation

Preparation & Storage

• Store at -20°C in small aliquot for long term storage. Avoid freeze/thaw cycle of the reagent.
• Shipped at 2-8°C. Store at 2-8°C for short term (1 month).
• The polyclonal antibody was purified by Protein A.
• The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.