Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody (100 μg)

Description

The rabbit polyclonal antibody specifically binds to the scFv region of a B-cell maturation antigen (BCMA) specific mouse monoclonal antibody (mAb, clone C11D5.3). BCMA is a protein that has been reported to be selectively expressed by B-lineage cells including multiple myeloma cells and restrictively expressed in both normal and malignant plasma cells at high levels. The scFv region of C11D5.3 has been used to develop BCMA-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-BCMA CAR expression on Jurkat cells. Jurkat cells were transduced with lentivirus encoding anti-BCMA CAR and cultured. 2×10⁵ cells were stained for the expression of anti-BCMA CAR with Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody (Product No. 500519, right panel). Secondary staining was carried out with Goat anti-Rabbit IgG (H+L), Alexa Fluor 647 (Product No. 700002). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel). Acquisition of >10, 000 events was performed.

Product Infomation

Preparation & Storage

• Store at -20°C in small aliquot for long term storage. Avoid freeze/thaw cycle of the reagent.
• Shipped at 2-8°C. Store at 2-8°C for short term (1 month).
• The polyclonal antibody was purified by Protein A.