Rabbit Anti-Leu16 Monoclonal Antibody (Clone L16-1G12) (100 µg)

Description

The rabbit monoclonal antibody L16-1G12 specifically binds to the scFv region of a CD20-specific mouse monoclonal antibody (mAb, clone Leu16). CD20 (cluster of differentiate 20) is a protein that is expressed on the surface of B cells, starting at the pre-B cell stage and also on mature B cells in the bone marrow and in the periphery. CD20 is not expressed on hematopoietic stem cells, pro-B cells, or normal plasma cells. The scFv region of Leu16 has been used to develop CD20-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Flow cytometric analysis of anti-CD20 CAR expression on human cell line Jurkat cells. Jurkat cells were transduced with lentivirus encoding anti-CD20 CAR and cultured. 2×10⁵ cells were stained for the expression of anti-CD20 CAR with Rabbit Anti-Leu16 Monoclonal Antibody (Product No. 219219, right panel). Secondary staining was carried out with AffiniPure F(ab’)2 Fragment Goat anti-Rabbit IgG(H+L), Alexa Fluor 647 (Product No. 700002). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).

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Preparation & Storage

• Store undiluted at -20°C.
• Avoid freeze/thaw cycle of the reagent.
• The monoclonal antibody was purified by Protein A.